I think Bowl of Red has it higher than the number I'd read, but that was 63 games, not 65. For 63, it's a mere 9.3 quintillion to 1. (that's 9.3 billion billion).
And yes, there's some basketball and particularly historical basketball knowledge to it. Like the number of 16 seeds who have advanced so far - zero. And while 15s have made it through, especially Florida Gulf Coast in recent memory, they're not that common. But if you bet straight "favorites" based on the seedings I've read somewhere that there's never been a tournament that panned out accordingly.
While picking #1 and maybe even #2 in the first round is a pretty sure bet in most years, it gets closer to a coin toss as you go to 14 v 3, 13 v 4, and the infamous 12 v 5. (Infamous because there have been more 12s upsetting 5s than there have been 11s upsetting 6 an anomalous statistic that will likely iron itself out when there is a larger data set, as right now from '85 to '13, there have only been 112 such matchups.)
But, once you have all that figured out, of course you have to guess WHICH 12 is going to pull the upset. And you should likely pay no attention to the 1985 tournament. In the opening round, 2 number 11s, 1 number 12, and 1 number 13 all upset heavily favored opposition.
Does knowing all this make me any better at playing the pools? No. In a really good year I might get 45 out of 63 right, but have never hit 50. And that's playing two or more pools a year.